Concentration Assay:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Western Blot:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Immunohistochemistry:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Chromatin Immunoprecipitation:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Ubiquitin Proteomics:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Control:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Transfection:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
Liposomes:Article Title: Disruption of tumor-intrinsic PGAM5 increases anti-PD-1 efficacy through the CCL2 signaling pathway
Article Snippet: .. Primary antibodies and reagents used in this study Antibody Concentration Application Identifier Company PGAM5 1:1000 WB MA5-24616 Invitrogen 1:200 IHC, IP ab126534 Abcam CD206 1:200 IHC 24595 CST CD86 1:200 IHC 91882 CST CD8 1:100 IHC ab217344 Abcam Hep-par1 1:200 IHC sc-58693 Santa cruz DRP1 1:1000 WB 8570 CST 1:100 IHC, IP ab56788 Abcam MFN1 1:1000 WB 66776-1-Ig Proteintech MFN2 1:1000 WB 67487-1-Ig Proteintech OPA1 1:1000 WB 66583-1-Ig Proteintech β-actin 1:1000 WB 66009-1-Ig Proteintech TLR9 1:200 IF, IHC 13674 CST dsDNA 1:200 IF ab27156 Abcam cGAS 1:200 IF 26416-1-AP Proteintech CCL2 1:200 IHC HPA019163 Sigma-Aldrich GAPDH 1:1000 WB 2118 CST LAMIN A/C 1:1000 WB 4777 CST NFκB 1:100 ChIP 8242 CST 1:1000 WB 8242 CST TRIM28 1:1000 WB 66630-1-Ig Proteintech Ubiquitin 1:1000 WB 3936 CST Flag 1:100 IP 14793 CST 1:1000 WB 14793 CST HA 1:1000 WB Ab9110 Abcam His 1:1000 WB 12698 CST Rabbit mAb IgG Isotype Control 1:100 ChIP, IP 3900 CST Goat anti-rabbit IgG AF594 1:200 IF ab150080 Abcam Goatanti-mouse IgG AF488 1:200 IF ab150113 Abcam Mitotracker 50nM IF M7512 Invitrogen Picogreen 3μl/ml IF P7581 Invitrogen E6446 10μM - HY-12756 MCE DNase I 5μg - HY-P72974 MCE Protein transfection reagent 10μl - HY-K2016 MCE PDTC 100μM - HY-18738 MCE MG132 20μM - HY-13259 MCE CHX 20/100μM - HY-12320 MCE In vivoMAb anti-mouse PD-1 mAb - - BE0146 BioXcell control liposomes - - F70101-A-10 FormuMax clodronate liposomes - - F70101C-A-10 FormuMax Table S5. ..
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